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Production of biologically active thymulin in Escherichia coli through expression of a chemically synthesized gene

✍ Scribed by Alphonse Calenda; Agnès Cordonnier; Florence Lederer; Diep K. H. Le; Jean Marie Pleau

Book ID
105086086
Publisher
Springer Netherlands
Year
1988
Tongue
English
Weight
532 KB
Volume
10
Category
Article
ISSN
0141-5492

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Enzymatically active D 5 -3-ketosteroid isomerase (KSI) protein with a C-terminus his 6 -tag was produced following insoluble expression using Escherichia coli. A simple, integrated process was used to extract and purify the target protein. Chemical extraction was shown to be as effective as homogen