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Production of antibodies that inhibit the binding of insulin to its receptor

โœ Scribed by Richard A. Roth; K.Y. Wong; Betty A. Maddux; I.D. Goldfine

Book ID
118315986
Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
465 KB
Volume
101
Category
Article
ISSN
0006-291X

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In the presence of anti-insulin antibody, 2-to 3-fold enhancement of 1251-insulin binding to liver membranes was observed when binding was estimated by the radioactivity of 125I-insulin bound to the membrane pellets. However, after 125I-insulin was covalently cross-linked to liver membranes using di