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Production of an enzymatic active protein using a continuous flow cell-free translation system

✍ Scribed by Yaeta Endo; Shiro Otsuzuki; Kimio Ito; Kin-ichiro Miura

Book ID
119131814
Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
678 KB
Volume
25
Category
Article
ISSN
0168-1656

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Cell-free synthesis of preparative amounts of interleukin-6 (IL-6) in a wheat germ extract is described. Following transcription of uncapped mRNA by SP6 polymerase, protein was synthesized in a continuous flow of translation components. The system remained active for at least \(50 \mathrm{~h}\), pro