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Production of an anti-MUC1 C595 dbFv antibody fragment in recombinant Escherichia coli

✍ Scribed by John Chi-Wei Lan; Tau Chuan Ling; Grant Hamilton; Andrew Lyddiatt


Book ID
108252575
Publisher
Elsevier Science
Year
2007
Tongue
English
Weight
509 KB
Volume
42
Category
Article
ISSN
1873-3298

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Fed-batch fermentation for production of a single-chain Fv antibody fragment (scFv) expressed as a recombinant periplastic protein from Escherichia coli was investigated. A high cell density of 50 g dry cell weight per liter was routinely achieved in a 14-L vessel by controlled exponential feeding o