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Production of an active recombinant human N-acetylgalactosamine-6-sulfate sulfatase enzyme in Pichia pastoris

✍ Scribed by Almeciga, Carlos J.; Rodríguez-López, Alexander; Sánchez, Jhonnathan; Moreno, Jefferson; Diaz, Dennis; Beltran, Laura M.; Espejo, Angela J.; Ruiz, Freddy O.; Barrera Pontificia, Luis A.


Book ID
122113279
Publisher
Elsevier Science
Year
2014
Tongue
English
Weight
47 KB
Volume
111
Category
Article
ISSN
1096-7192

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✍ Hiroshi Nakakubo; Hajime Fukuyama; Masahide Nakajima; Teruaki Imada; Shusei Uno; 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 140 KB 👁 1 views

We succeeded in expressing in a Pichia pastoris (P. pastoris) host a cDNA encoding a mature human chymase (h-chymase) which was secreted directly into the culture medium. Recombinant human heart chymase (rh-chymase) was purified from the culture medium via a single one-step heparin-agarose column ch