Production of Acinetobacter radioresistens lipase with repeated batch culture in presence of nonwoven fabric

✍ Scribed by Ying-Chien Lin; Jau-Yann Wu; Teh-Liang Chen

• Book ID: 101720797
• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 141 KB
• Volume: 76
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.1185

✦ Synopsis

Abstract

Cultivation of Acinetobacter radioresistens on n‐hexadecane for lipase production was investigated with repeated batch culture in the presence of a hydrophobic nonwoven fabric. Lipase production followed the growth‐associated model, and the repeated batch culture could achieve both high enzyme yield and increased volumetric productivity. The fabric was shown to be able to disperse n‐hexadecane, to adsorb the unused hydrocarbon, and to retain bioemulsifiers excreted from the cells; therefore, it enhanced cell growth and, in turn, lipase production. In the repeated batch culture in the absence of the fabric, lipase yield and volumetric productivity were found to be 21 U/mL and 875 U/L · h, respectively. However, if the fabric was equipped in the fermentor, lipase yield and volumetric productivity increased to 30 U/mL and 2500 U/L · h, respectively. The lipase production profile could be further improved by raising the amount of nitrogen source and, as a result, a lipase yield of 54 U/mL and a volumetric productivity of 2250 U/L · h were obtained. In this study we assess the beneficial effects of nonwoven fabric on lipase production. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 76: 214–218, 2001.