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Production of a thermophilic, extracellular alkaline protease byBacillus stearothermophilusAP-4

✍ Scribed by R. Dhandapani; R. Vijayaragavan

Publisher
Springer
Year
1994
Tongue
English
Weight
225 KB
Volume
10
Category
Article
ISSN
1573-0972

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✦ Synopsis

A thermophilic Bacillus stearothermophilus strain AP-4 excreting a thermostable alkaline protease, was isolated from a local compost. Maximum activity of protease (250 U/ml) was after 36 h growth in broth at pH 9.0 and at 55Β°C. The protease was optimally active at pH 9.0 and 55Β°C and was stable in 5 mM CaCl 2. The enzyme was completely inactivated by PMSF, EDTA and ]/-mercaptoethanol. It is therefore a metal ion-dependent, alkaline, serine protease.

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Production of thermostable alkaline lipa
Production of thermostable alkaline lipase on vegetable oils from a thermophilic Bacillus sp. DH4, characterization and its potential applications as detergent additive
✍ Limpon Bora; Mohan C Kalita πŸ“‚ Article πŸ“… 2008 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 132 KB

## Abstract **BACKGROUND:** The alkaline lipase production on vegetable oils as sole carbon source, its characterization and effect of different commercial detergents and surfactants on enzymatic activity from thermophilic __Bacillus__ sp. DH4 was investigated. **RESULTS:** The organism grew on ma