✍ Scribed by Walda B van Zyl; Gert H.J Pretorius; Manfred Hartmann; Harry F Kotzé
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
Absstract: Maintaining a metabolically productive state for recombinant Escherichia coli remains a central problem for a wide variety of growth-dependent biosynthesis. This problem becomes particularly acute under conditions of minimal cell growth such as fed-batch fermentations. In this, we investi
A genetic strategy to enhance recombinant protein production is discussed. A small DNA bending protein, Fis, which has been shown to activate rRNA synthesis upon a nutrient upshift, was overexpressed in E. coli strain W3110 carrying vector pUCR1. Overexpres- sion of Fis during exponential growth was
Scientists across disciplines have increasingly come to recognize the power of the protein. Current Protocols in Protein Science on CD-ROM (CPPS on CD) was developed in response to this revitalized interest and provides the most comprehensive collection of expert protein methods available. The publi
The Escherichia coli mutant CWML2 was previously reported to exhibit improved physiological characteristics, including recombinant protein production. Here we investigate the molecular basis of this phenotype by comparing the cellular level of three RNA polymerase sigma subunits by immunoblot analys