Production of a plasmin inhibitory substance by Scopolia japonica suspension cultures

✍ Scribed by Masanaru Misawa; Hozumi Tanaka; Osamu Chiyo; Noboru Mukai

• Publisher: John Wiley and Sons
• Year: 1975
• Tongue: English
• Weight: 414 KB
• Volume: 17
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.260170302

✦ Synopsis

Abstract

A potent inhibitory agent against human plasmin, fibrinolytic proteinase, has been found in the extracts of callus tissue of Scopolia japonica. Effects of cultural conditions on cell growth and production of the plasmin inhibitory substance by this cell line in suspension cultures were examined in MurashigeSkoog's medium. More than l.5 mg of the inhibitor, as t‐amino cyclohexane carboxylic acid, a synthetic plasmin inhibitor, were observed to accumulate per ml of medium containing 0.83 g of NH~4~NO~3~ and 7.6 g of KNO~3~ per liter as well as suitable levels of growth hormones.

Addiction of antibiotics and deformers were examined in preliminary tests for large scale cultivation. Semicontinuous culture on a small scale in a glass cylinder, was also tested and growth rate of 1.29 g/liter/day (by dry wt) was obtained. Plasmin inhibitory activities in the extracts of the results intact plant and in cultured cells of S. japonica were compared and the results indicated that cell suspension culture was superior to extraction the natural plant for inhibitor production.