๐”– Bobbio Scriptorium
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Production of a monoclonal antibody directed against the nerve growth factor receptor from sympathetic membranes

โœ Scribed by Claudia J. Morgan; Ralph A. Bradshaw

Publisher
John Wiley and Sons
Year
1985
Tongue
English
Weight
737 KB
Volume
27
Category
Article
ISSN
0730-2312

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โœฆ Synopsis

Spleen cells from BALB/c mice immunized with a plasma membrane-enriched fraction from rabbit sympathetic ganglia were Sused with the mouse myeloma NS 1. A hybrid clone was obtained that produced monoclonal antibody directed against the receptor for nerve growth factor (NGF). The antibody, identified as IgC, was able to immunoprecipitate solubilized NGF receptor in the presence or absence of bound NGF. The antibody bound specificall to sympathetic membranes with high affinity but did not affect the binding of Y'sI-NCiF to its receptor in sympathetic or sensory neurons or PC12 cells.

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โœ M. Clagett-Dame; C. Chung; M. V. Chao; P. S. DiStefano ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 921 KB

## Abstract Monoclonal antibodies (designated IIIG5, VIID1, VIIIC8, and XIF1) have been produced that bind to the human nerve growth factor receptor (NGFโ€R) as well as to a soluble, truncated form of the receptor (NGFโ€Rt). The antibodies were generated against partially purified NGFโ€Rt from the con