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Production of a lipopeptide antibiotic, surfactin, by recombinant Bacillus subtilis in solid state fermentation

✍ Scribed by Akihiro Ohno; Takashi Ano; Makoto Shoda

Publisher: John Wiley and Sons
Year: 1995
Tongue: English
Weight: 577 KB
Volume: 47
Category: Article
ISSN: 0006-3592
DOI: 10.1002/bit.260470212

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✦ Synopsis

Production of a lipopeptide antibiotic, surfactin, in solid state fermentation (SSF) on soybean curd residue, Okara, as a solid substrate was carried out using Bacillus subtilis MI113 with a recombinant plasmid pC112, which contains lpa-14, a gene related to surfactin production cloned at our laboratory from a wild-type surfactin producer, B. subtilis RB14. The optimal moisture content and temperature for the production of surfactin were 82% and 37"C, respectively. The amount of surfactin produced by MI113 (pC112) was as high as 2.0 g/kg wet weight, which was eight times as high as that of the original B. subtilis RBI4 at the optimal temperature for surfactin production, 37°C. Although the stability of the plasmid showed a similar pattern in both SSF and submerged fermentation (SMF), production of surfactin in SSF was 4-5 times more efficient than in SMF.

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