Production of 2,3-butanediol from glucose by GRAS microorganism Bacillus amyloliquefaciens

✍ Scribed by Taowei Yang; Zhiming Rao; Xian Zhang; Qing Lin; Haifeng Xia; Zhenghong Xu; Shangtian Yang

• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 223 KB
• Volume: 51
• Category: Article
• ISSN: 0233-111X
• DOI: 10.1002/jobm.201100033

✦ Synopsis

Abstract

In the current study, a GRAS (Generally Recognized As Safe) strain of Bacillus amyloliquefaciens producing 2,3‐butanediol (2,3‐BD) designated as B10‐127 was isolated in our lab. The strain B10‐127 produced 2,3‐BD effectively under the condition of 20% glucose (quality concentration), showed a high‐glucose tolerance. The effects of initial glucose concentration, temperature, pH and agitation on 2,3‐BD production were investigated in this work and the proper parameters were identified. Accordingly, the fed‐batch culture of B10‐127 in larger scales (5 l) showed a remarkable 2,3‐BD producing potency. The maximum 2,3‐BD concentration reached 92.3 g/l at 96 h with a 2,3‐BD productivity of 0.96 g/l h. To our knowledge, the results were new records on 2,3‐BD fermentation by Bacillus, which shown an excellent candidate for the microbial fermentation of 2,3‐BD on an industrial scale. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)