Production of 2-ketobutyric acid from 1,2-butanediol by resting cells ofRhodococcus equiIFO 3730

✍ Scribed by Tadaatsu Nakahara; Toshiaki Nakajima-Kambe; Shinji Sato

• Book ID: 104635545
• Publisher: Springer Netherlands
• Year: 1994
• Tongue: English
• Weight: 346 KB
• Volume: 16
• Category: Article
• ISSN: 0141-5492
• DOI: 10.1007/bf00134623

✦ Synopsis

Various microor f

anisms were screened for their ability to mduce 2-ketobut R ric acid (2-KBA) from ,Zbutanediol (1,2-BD).

Among them, R o&xuc~us equi ft FO 3730 was selected as the best strain. The various culture and reaction conditions were optimized using this strain. Limitation of thiamine in the to be effective. The resting cells of the strain grown on 1, T owing medium was found -pro source yielded 15.7 g!l of 2-KBA from 20 g/l of 1,2-BD after B anediol as the carbon 2 h incubation at 30°C in the reaction mixture under optimal conditions.