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Production, Extraction, and Purification of Human Poly(ADP-ribose) Polymerase-1 (PARP-1) with High Specific Activity

✍ Scribed by Matthew I. Knight; Paul J. Chambers


Book ID
115645923
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
158 KB
Volume
23
Category
Article
ISSN
1046-5928

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## Abstract Poly(ADP‐ribose) polymerase‐1 (PARP‐1) is a mammalian enzyme that attaches long branching chains of ADP‐ribose to specific nuclear proteins, including itself. Because its activity __in vitro__ is dependent upon interaction with broken DNA, it has been postulated that PARP‐1 plays an imp