Production and synthetic dyes decolourization capacity of a recombinant laccase from Pichia pastoris
β Scribed by L. Lu; M. Zhao; S.-C. Liang; L.-Y. Zhao; D.-B. Li; B.-B. Zhang
- Book ID
- 108840509
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 263 KB
- Volume
- 107
- Category
- Article
- ISSN
- 1364-5072
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## Abstract __Pichia pastoris,__ a methylotrophic yeast, is an efficient producer of recombinant proteins in which the heterologous gene is under the control of the methanolβinduced __AOX1__ promoter. Hence, the accepted production procedure has two phases: In the first phase, the yeast utilizes gl
Pichia pastoris strains with capability to sequentially express two recombinant proteins under control of the constitutive glyceraldehydes-3-phosphate dehydrogenase (GAP) and the methanol-induced alcohol oxidase (AOX1) promoters have been constructed. The intracellular β€-galactosidase (β€-gal), secre