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Production and secretion of proteolytic enzymes by normal and neoplastic cells

✍ Scribed by J. Barry Boyd; Roderick M. Farb; Fred J. Yost Jr; Nicholas Georgiade; Gerald S. Lazarus

Publisher
John Wiley and Sons
Year
1979
Tongue
English
Weight
440 KB
Volume
11
Category
Article
ISSN
0022-4790

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✦ Synopsis

A possible mechanism for tumor cell invasion of normal tissue might be secretion of proteolytic enzymes. This study compares and contrasts production and secretion of proteinases by cell cultures of normal and chemically transformed mouse epithelial cells. Lysates of normal and neoplastic cells contain similar amounts of neutral proteinase, cathepsin D and plasminogen activator.

Neither collagenase nor elastase could be identified in lysates of, or serum-free culture medium bathing, normal or neoplastic cells. Neoplastic cells secrete ten times more plasminogen activator than normal cells. Our data support the hypothesis that plasminogen activator produced by neoplastic cells could function to activate latent proteolytic enzymes secreted by connective tissue cells which might result in spread of neoplastic cells into normal tissue.

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