Production and characterization of antibodies to surface antigens of human neuroblastomas
- Book ID
- 123118722
- Publisher
- Elsevier Science
- Year
- 1982
- Weight
- 174 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0277-5379
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MAb were derived from mice immunized with cells of the human neuroblastoma line IMR-32. Five hybridomas were selected according to their selective binding to human cell lines, tumors and normal tissues. One of them, CE7, reacted with all sympatho-adrenomedullary cells (neuroblastoma, ganglioneurobla
## Abstract Hybridoma CE7 produces a murine antibody (yl/k) which binds to a 190‐kDa cell‐surface glycoprotein of human neuroblas‐toma. Because of its tumor specificity, it has been used routinely in clinical pathology to confirm diagnosis of neuroblastoma. We have Isolated the gene segments coding
## Abstract Sera of 200 non‐transfused healthy male blood donors were tested for antibody reactivity to cell‐surface antigens of cultured astrocytoma cells. Positive reactions were observed only rarely by protein‐A assay (PA), in about half the cases by immune adherence assay (IA) and in nearly all