Production and characterization of a xylanase fromCyathus stercoreus
โ Scribed by A. Saxena; R. C. Kuhad; R. K. Saxena; R. Gupta
- Publisher
- Springer
- Year
- 1994
- Tongue
- English
- Weight
- 187 KB
- Volume
- 10
- Category
- Article
- ISSN
- 1573-0972
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โฆ Synopsis
Cyathus stercoreus grown on wheat straw had a higher xylanase activity than when it was grown on rice husk or extracted hemicellulose. Inclusion of casein hydrolysate, Tween 80 and Mn ~รท (at 0.02%, 0.2% and 0.075%, respectively) increased the production of extracellular xylanase. Optimal yield of xylanase (0.73 U/ml) was at pH 5.6 after 9 to 12 days at 30ยฐC. The xylanase was stable at pH 4.5 to 7.5 for 2h but above 50ยฐC its stability fell sharply.
๐ SIMILAR VOLUMES
Alkali-treated corn stalk gave maximum xylanase production at supporting growth of Streptomyces HM-15. Xylanase was stable for 24 h over a pH range of 5.0 to 7.0, had optimal activity between 50 and 60ยฐC and a halflife of 5 h at 60ยฐC. Xylanase production and activity were inhibited by xylose.