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Production and characterization of a polyclonal antibody against an actin peptide

✍ Scribed by Nazila Amini; Elham Safarpur; Reza Hadavi; Hodjattallah Rabbani; Ahmad-Reza Mahmoudi; Ali-Ahmad Bayat; Jafar Mahmoudian; Mohaddeseh Naghi-Vishteh; Jeddi-Tehrani Mahmood


Book ID
118430599
Publisher
Elsevier Science
Year
2011
Tongue
English
Weight
59 KB
Volume
44
Category
Article
ISSN
0009-9120

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Characterization of a monoclonal antibod
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Anti-actin monoclonal antibodies were prepared using phalloidin-stabilized actin that was purified from pea roots by DNase I affinity chromatography. One monoclonal antibody, designated mAb3H 1 1, bound plant actin in preliminary screenings and was further analyzed. Immunoblot analysis showed that t