Processing small tissue specimens in acrylic resins for ultramicrotomy: Improved handling and orientation
✍ Scribed by Ridgway, Richard L. ;Chestnut, Matthew H.
- Book ID
- 102332304
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1984
- Tongue
- English
- Weight
- 142 KB
- Volume
- 1
- Category
- Article
- ISSN
- 0741-0581
No coin nor oath required. For personal study only.
✦ Synopsis
Methods i n t r o d u c e d t o a c h i e v e such o r i e n t a t i o n have been d e s i g n e d p r i m a r i l y f o r use w i t h epoxy r e s i n s . Gaining i n p o p u l a r i t y , however, a r e a number o f water-miscible low v i s c o s i t y a c r y l i c r e s i n s (e.g., g l y c o l methacrylate, L.R. White, etc.) having p o t e n t i a l a p p l i c a t i o n s i n immunocytochemical , enzyme histochemical, and X-ray m i c r o a n a l y t i c a l s t u d i e s . WP present. h e r e a mathod f o r p r o c e s s i n g s m a l l t i s s u e specimens i n a c r y l i c r e s i n s t h a t combines t h e w e l l known technique o f agar pre-embedding fsee S c o t t -e t a l , 1970) w i t h t h e use o f a p o l y e s t e r s u p p o r t p l a t f o r m . The disc-shaped p l a t f o r m serves as a specimen c a r r i e r d u r i n g p r o c e s s i n g and, t o g e t h e r w i t h t h e a g a r s u p p o r t , a i d s i n m i n t a i n i n g specimen o r i e n t a t i o n d u r i n g r e s i n polymerization. A f t e r t h e r e s i n i s cured, removal of t h e d i s c p r o v i d e s a p r e d i c t a b l e p l a n a r s u r f a c e r e l a t i v e t o the specimen, which f a c i l i t a t e s t h e trimming of b l o c k faces.
MATERIALS 4ND METHODS
P o l y e s t e r s u p p o r t p l a t f o r m s 3 r e prepared by f i r s t developing a sheet o f unexposed photographic f i l m t o remove i t s e m u l s i o n , and t h e n p u n c h i n g d i s c s f r o m t h i s m a t e r i a l u s i n g a standard h o l e punch. We have used Kodak --