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Processing Properties of Recombinant Human Procathepsin L

✍ Scribed by Toshiyuki Nomura; Yukio Fujisawa


Book ID
115579601
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
213 KB
Volume
230
Category
Article
ISSN
0006-291X

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Human procathepsin L has been expressed in the yeast Pichiapastoris and its inactive (Cys25Ser) and unglycosylated (ThrllOAla) mutant purified, concentrated to 4 mglml, and crystallized by vapor diffusion against solution containing 1.4 M (Na,K)PO, buffer, pH 7.8. Crystal size was increased by multi