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Processing of plasmid DNA with ColE1-like replication origin

✍ Scribed by Zhijun Wang; Zhenghong Yuan; Ulrich R. Hengge


Book ID
113855168
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
190 KB
Volume
51
Category
Article
ISSN
0147-619X

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Approximately 200,000 clones of Escherichia coli carrying mutagen-treated colicinogenic plasmid E1 (ColE1) were examined for irreversible loss of the plasmid at 43 degrees. Thirty of these clones that appeared to be most defective in plasmid DNA replication at the non-permissive temperature were sel