Processing biological images from very low light emissions

✍ Scribed by Bernroider, Gustav

• Publisher: John Wiley and Sons
• Year: 1994
• Weight: 673 KB
• Volume: 9
• Category: Article
• ISSN: 0884-3996
• DOI: 10.1002/bio.1170090305

✦ Synopsis

As opposed to strong light signals (> 10' photons mm-' s-'), the construction of images from object sources with low level signals (< 10' photonsmrn-'s-') involves a probabilistic transfer-or point-spread function. The resulting images carry considerable uncertainty or spread, restricting resolution and quantification. In this paper w e propose various strategies how to reconstruct object characteristics from very low light emissions by unfolding the imaging equation. Further, calibration techniques which help t o associate light emissions with the decomposition of luminogenic substrates in a spatially selective way will be discussed.