This work demonstrates that proper selection of a metal ion and chelating ligand enables recovery of a his(6)-tagged protein from canola (Brassica napus) extracts by immobilized metal affinity chromatography (IMAC). When using Co(2+) with iminodiacetate (IDA) as the chelating ligand, beta-glucuronid
Process Development for Antibody Purification from Tobacco by Protein A Affinity Chromatography
β Scribed by C. Hey; C. Zhang
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 611 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0930-7516
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