Problems in the application of gel filtration to the desalting of organic compounds: Retardation of aromatic and heteroaromatic anions by commonly used salts
✍ Scribed by Paul C. Engel
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 624 KB
- Volume
- 82
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
On Bio-Gel P-2 columns, certain inorganic salts elute considerably later than nucleotides and other anionic compounds of similar molecular weight when they are run separately. This would suggest that such compounds should be readily desalted by gel filtration. With some salts (e.g., NaCl, KI, KSCN) this is indeed the case. With others, however(K,HPO,, CH,COONa, HCOONH,), there is complete overlap between the peaks of the salt and the organic compound. Studies with different organic compounds and salts suggest that this entrapment within the salt peak may be due to opposing electrostatic and hydrophobic influences on the mobility of organic anions.