✍ Scribed by Billaudelle, H. ;Hedén, C.-G. ;Malmgren, B.
No coin nor oath required. For personal study only.
A study of factors affecting the reproducibility and yields in cultivations of H . pertussis (the whooping cough organism). The search for suitable media led to the development of a new kind of blood medium suitable for pre-cultures. Sterility of the blood additive could be ensured by filtration, and contamination on starting the freeze-dried cells was avoided by the use of a special kind of ampoule.
In the course of studies of different cultivation techniques a 6,000 ml 'pulsaerator' was found t o be ideal for the preparation of ordinary small batches. Inoculation density, aeration and other factors affecting the yield were studied and a culture cycle suitable for preparing 500 1. batches was developed.
* The research reported here has been made possible through the support and sponsorship of the U.S. Department of the Army, through its European Research Office.
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