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Problem-solving test: In vitro protein kinase a reaction

✍ Scribed by József Szeberényi

Publisher
The American Society for Biochemistry and Molecular Biology
Year
2009
Tongue
English
Weight
43 KB
Volume
37
Category
Article
ISSN
1470-8175

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A simple calculation using the radioactive decay of (32)P incorporated into a protein during in vitro kinase reactions is described that allows the overall stoichiometry of phosphorylation for the substrate protein or peptide to be calculated. Prior to using techniques such as diagnostic ion scannin