Problem-solving Test: DNA manipulation with alkaline phosphatase and polynucleotide kinase

Purification of a polynucleotide kinase

Purification of a polynucleotide kinase from calf thymus, comparison of its 3′-phosphatase domain with T4 polynucleotide kinase, and investigation of its effect on DNA replication in vitro

✍ Arshad Jilani; Carolyn Slack; Diamanto Matheos; Maria Zannis-Hadjopoulos; Dana D 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 148 KB 👁 1 views

Mammalian polynucleotide kinases (PNKs) carry out 5Ј-phosphorylation of nucleic acids. Although the cellular function(s) of these enzymes remain to be delineated, important suggestions have included a role in DNA repair and, more recently, in DNA replication. Like T4 PNK, some preparations of mammal