Problem in liquid scintillation counting: Adsorption of [14C]polyethylene glycol in dilute solutions

✍ Scribed by William G. Crouthamel; Knox Van Dyke

• Publisher: Elsevier Science
• Year: 1975
• Tongue: English
• Weight: 517 KB
• Volume: 66
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(75)90741-1

✦ Synopsis

Wlpolyethylene glycol is the method of choice for quantitating changes in intestinal water flux during drug absorption experiments in animals and man. This study points out some of the problems which can be encountered in using this method and provides ways to minimize these problems. Polyethylene glycol selectively binds to the glass wall of scintillation vials during counting and results in a decrease in counting efficiency as a function of time. The results obtained when using this method are determined by the choice of scintillation vial, scintillation cocktail, concentration of polyethylene glycol and the time period over which the samples are counted. 234