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Probing the Role of Backbone Hydrogen Bonding in a Critical β Sheet of the Extracellular Domain of a Cys-Loop Receptor

✍ Scribed by Kristin R. Gleitsman; Henry A. Lester; Dennis A. Dougherty

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
606 KB
Volume
10
Category
Article
ISSN
1439-4227

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TGF-␤ is a ubiquitous protein that exhibits a broad spectrum of biological activity. The prokaryotic expression and purification of the extracellular domain of the type II TGF-␤ receptor (T␤R-II-ED), without the need for fusion protein cleavage and refolding, is described. The recombinant T␤R-II-ED