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Probing the regulatory site of Escherichia coli aspartate transcarbamoylase by site-specific mutagenesis

โœ Scribed by Zhang, Yang; Kantrowitz, Evan R.

Book ID
126000046
Publisher
American Chemical Society
Year
1992
Tongue
English
Weight
911 KB
Volume
31
Category
Article
ISSN
0006-2960

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โœ Jin-Tann Chen; Ruth J. Mayer; Carol A. Fierke; Stephen J. Benkovic ๐Ÿ“‚ Article ๐Ÿ“… 1985 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 441 KB

Two site-specific mutations of dihydrofolate reductase from Escherichiu coli based on the x-ray crystallographic structure were constructed. The first mutation (His-45 + Gln) is aimed at assessing the interaction between the imidazole moiety and the pyrophosphate backbone of NADPH. The second (Thr-1