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Probing the Nature of Noncovalent Interactions by Mass Spectrometry. A Study of Protein−CoA Ligand Binding and Assembly

✍ Scribed by Robinson, Carol V.; Chung, Evonne W.; Kragelund, Birthe B.; Knudsen, Jens; Aplin, Robin T.; Poulsen, Flemming M.; Dobson, Christopher M.

Book ID
121181003
Publisher
American Chemical Society
Year
1996
Tongue
English
Weight
284 KB
Volume
118
Category
Article
ISSN
0002-7863

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A novel mass spectrometry-based methodology using electrospray ionization (ESI) is described for the detection of protein-protein [interferon (IFN)-γ dimer] and protein-ligand [ras-guanosine diphosphate (GDP)] noncovalent interactions. The method utilizes ESI from aqueous solution at appropriate pH.