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Probing the Domain Structure of Abrin-a by Tryptic Digestion

✍ Scribed by Song-How Lin; Lu-Ping Chow; Yung-Liang Chen; Yen-Chywan Liaw; Jeen-Kuan Chen; Jung-Yaw Lin


Book ID
115132150
Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
592 KB
Volume
240
Category
Article
ISSN
1432-1327

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We set up a method to get rapid information on the three-dimensional structure of peptide and proteins of known sequence. Both native and alkylated polypeptide is hydrolyzed with a number of proteases at different digestion times and the resulting mixtures are compared by HPLC analysis to establish