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Probing the Activator and Hydrophobic Substrate Binding Sites of PTEN by 31P NMR

✍ Scribed by Wei, Yang; Roberts, Mary F.


Book ID
122198300
Publisher
Biophysical Society
Year
2012
Tongue
English
Weight
37 KB
Volume
102
Category
Article
ISSN
0006-3495

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Proton decoupled 31P and 13C solution NMR experiments were applied to mixtures of 3-deoxy-D-manno-2-octulosonate-8-phosphate (KDO8P) synthase, with each of its natural substrates, phosphoenolpyruvate and arabinose-5-phosphate (ASP), and product KDO8P to identify the formation of the enzyme-substrate