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Probing protein function by chemical modification

✍ Scribed by Yao-Wen Wu; Roger S. Goody

Book ID
105359916
Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
326 KB
Volume
16
Category
Article
ISSN
1075-2617

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✦ Synopsis

Abstract

Labeling proteins with synthetic probes, such as fluorophores, affinity tags, and other functional labels is enormously useful for characterizing protein function in vitro, in live cells, or in whole organisms. Recent advancements of chemical methods have substantially expanded the tools that are applicable to modify proteins. In this review, we discuss some important chemical methods for site‐specific protein modification and highlight the application of established techniques to tackle biological questions. Copyright Β© 2010 European Peptide Society and John Wiley & Sons, Ltd.

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## Abstract The spleen of the exhypoxic polycythemic mouse was employed as a model system to study the effect of erythropoietin on enzymes that chemically modify nuclear proteins. At selected time intervals after in vivo administration of erythropoietin, acetyltransferase and methyltransferase acti