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Probing Nucleocytoplasmic Transport with Fluorescence Fluctuation Spectroscopy and Two-photon Activation of Photoactivable GFP

✍ Scribed by Chen, Yan; Wu, Bin; Mueller, Joachim

Book ID
119205062
Publisher
Biophysical Society
Year
2009
Tongue
English
Weight
39 KB
Volume
96
Category
Article
ISSN
0006-3495

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## Abstract Two‐photon activation of photoactivatable green fluorescent protein (PA‐GFP) provides a unique tool for probing cellular transport processes, because activation is strictly limited to the subfemtoliter optical volume of the two‐photon spot. We demonstrate two‐photon activation of PA‐GFP