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Probing a tRNA core that contributes to aminoacylation

✍ Scribed by Christian S Hamann; Ya-Ming Hou


Book ID
115629865
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
406 KB
Volume
295
Category
Article
ISSN
0022-2836

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A Homogeneous Method to Measure Aminoacy
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A new method to measure the aminoacylation of tRNA based upon the use of the scintillation proximity assay (SPA) technology has been developed. The assay detects incorporation of radiolabeled amino acids into cognate tRNA, catalyzed by a specific aminoacyl-tRNA synthetase (aaRS). Under acidic condit