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Principles for the construction and operation of a device for rapidly freezing suspensions for cryo-electron microscopy

✍ Scribed by Murray, John M. ;Ward, Rob

Publisher: Wiley (John Wiley & Sons)
Year: 1987
Tongue: English
Weight: 581 KB
Volume: 5
Category: Article
ISSN: 0741-0581
DOI: 10.1002/jemt.1060050308

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✦ Synopsis

Details are given concerning the construction and use of a simple device for preparing samples of frozen-hydrated biological material embedded in thin films of vitreous ice. The control of humidity around the sample and rate of freezing are of prime importance to obtain good specimens for cryo-electron microscopy. Symptoms of ill performance of such an apparatus and poor cryo-manipulation are discussed.

Rapid freezing, Frozen-hydrated suspensions

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