Principal component analysis of mRNA levels of genes related to inflammation and fibrosis in rats treated with TNBS or glutamine
✍ Scribed by Jonghoon Kang; Ashlie K. Patterson
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 56 KB
- Volume
- 17
- Category
- Article
- ISSN
- 1078-0998
No coin nor oath required. For personal study only.
✦ Synopsis
Inflammatory bowel diseases such as Crohn's disease and ulcerative colitis are a major concern for human health. 1 However, they often lack effective treatments and therefore several attempts have been made to discover ways to treat these diseases. 2 In a recent article, San-Miguel et al 3 investigated the feasibility of glutamine as a therapeutic agent for such diseases using a rat model of colitis induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS). The major finding of the study is that glutamine treatment reduces the inflammatory response, suggesting glutamine could be a candidate for a therapeutic agent for Crohn's disease. We report our analysis of the study that could be valuable to the inflammatory bowel diseases research community.
While the article 3 clearly shows that glutamine modulates the expression of genes related to inflammation and fibrosis, we found that the gene expression patterns could be further analyzed using principal component analysis (PCA) to draw quantitative information. In this letter we show our analysis to draw such information, which is not apparent in the original article.
In the article, the authors report mRNA levels of 12 genes related to Copyright V C 2010 Crohn's & Colitis Foundation of America, Inc.