PRIMORDIAL GERM CELL-DERIVED MOUSE EMBRYONIC GERM (EG) CELLSIN VITRORESEMBLE UNDIFFERENTIATED STEM CELLS WITH RESPECT TO DIFFERENTIATION CAPACITY AND CELL CYCLE DISTRIBUTION
✍ Scribed by JÜRGEN ROHWEDEL; ULRICH SEHLMEYER; JIN SHAN; ARMIN MEISTER; ANNA M. WOBUS
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 588 KB
- Volume
- 20
- Category
- Article
- ISSN
- 1065-6995
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Embryonic germ (EG) cells of line EG‐1 derived from mouse primordial germ cells were investigated for their__in vitro__differentiation capacity. By cultivation as embryo‐like aggregates EG‐1 cells differentiated into cardiac, skeletal muscle and neuronal cells accompanied by the expression of tissue‐specific genes and proteins as shown by RT‐PCR analysis and indirect immunofluorescence. In comparison to embryonic stem (ES) cells of line D3 the efficiency of differentiation into cardiac and muscle cells was comparatively low, whereas spontaneous neuronal differentiation was more efficient than in D3 cells. Furthermore, the distribution of cell cycle phases as a parameter for the differentiation state was analysed in undifferentiated EG cells and ES cells and compared to data obtained for embryonic carcinoma (EC) cells of line P19 and differentiated, epithelioid EPI‐7 cells. Flow cytometric analysis revealed similar cell cycle phase distributions in EG, EC and ES cells. In contrast, the somatic differentiated EPI‐7 cells showed a longer G~1~‐phase and shorter S‐ and G~2~/M‐phases. Together, our results demonstrate that the differentiation state and capacity of EG cells__in vitro__resemble that of totipotent ES cells.