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Primer sets for cloning the human repertoire of T cell Receptor Variable regions

โœ Scribed by Ilenia Boria; Diego Cotella; Irma Dianzani; Claudio Santoro; Daniele Sblattero


Book ID
115005157
Publisher
BioMed Central
Year
2008
Tongue
English
Weight
387 KB
Volume
9
Category
Article
ISSN
1471-2172

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Conventional polymerase chain reactions (PCR) require sequence information on both ends of the DNA to be amplified. The novel technique described here allows the amplification of cDNA fragments with sequence information from one end only. Blunt-ended double-strand cDNA is prepared, circularized with