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Primer extension on microarrays for typing single-nucleotide polymorphisms and detecting disease-causing mutations

✍ Scribed by Pastinen, Tomi; Raitio, Mirja; Lindroos, Katarina; Niini, Paavo; Katila, Pekka; Peltonen, Leena; Syvänen, Ann-Christine


Book ID
109531374
Publisher
Nature Publishing Group
Year
1999
Tongue
English
Weight
113 KB
Volume
23
Category
Article
ISSN
1061-4036

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✍ Ann-Christine Syvänen 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 383 KB 👁 2 views

In the minisequencing primer extension reaction, a DNA polymerase is used specifically to extend a primer that anneals immediately adjacent to the nucleotide position to be analyzed with a single labeled nucleoside triphospate complementary to the nucleotide at the variant site. The reaction allows