Although serum-free media have been widely used in mammalian cell culture for therapeutic protein production, the effects of serum-substitutes on product quality have not been extensively examined. This study observed an adverse effect of Primatone RL, an animal tissue hydrolysate commonly used as a
Primatone RL in mammalian cell culture media
โ Scribed by A. Mizrahi
- Publisher
- John Wiley and Sons
- Year
- 1977
- Tongue
- English
- Weight
- 190 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0006-3592
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
## Abstract We sought a cultured cell line with Proline Oxidase activity to study the regulation and physiologic role of the enzyme in mammalian tissues. Among the cell lines tested, only LLCโRK~1~ cells, derived from rabbit kidney, had significant Proline Oxidase activity; the K~m~ for proline of
We present the setup of a flow injection analysis system designed for on-line monitoring of glutamate and glutamine. These amino acids represent a major energy source in mammalian cell culture. A cycling assay consisting of glutamate dehydrogenase and aspartate aminotransferase produces NADH proport