Primary structure of the variable regions of a monoclonal antibody MUSE11 recognizing the tandem repeat domain of a mucin core protein, MUC1

✍ Scribed by Dr. Yuji Hinoda; Yoshiaki Arimura; Fumio Itoh; Masaaki Adachi; Masayuki Tsujisaki; Kohzoh Imai; Akira Yachi

• Book ID: 102307790
• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 438 KB
• Volume: 7
• Category: Article
• ISSN: 0887-8013
• DOI: 10.1002/jcla.1860070206

✦ Synopsis

A monoclonal antibody (MAb) MUSE1 1 recognizes an epitope in the tandem repeat domain of a mucin core protein, MUCl. We show that the epitope of MAb MUSEl 1 could be within the continuous amino acid sequence PDTRPAPG. Since there is increasing evidence indicating that this region is highly immunogenic, cDNA cloning of the variable regions of heavy-chain (VH) and of lightchain (VL) of MAb MUSEl 1 was performed by using RT-PCR to provide a basis for analyzing the structure of the antibody-antigen complex and for producing anti-idiotypic antibodies. The deduced amino acid sequence revealed that the VH and VK of MAb MUSE1 1 could be assigned to subgroups IllA and II of mouse immunoglobulin heavy and light chains, respectively. When compared with the V regions of other MAbs in the same subgroup, the complementary determining region 3 (CDR3) in the VH region of MAb MUSEl 1 consisted of a unique sequence that may be important in defining the specificity of MAb MUSE1 1.