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Primary structure and expression pattern of the 33-kDa chitinase gene from the mycoparasitic fungusTrichoderma harzianum

✍ Scribed by M. Carmen Limón; José M. Lora; Irene García; Jesús Cruz; Antonio Llobell; Tahía Benítez; José A. Pintor-Toro


Publisher
Springer-Verlag
Year
1995
Tongue
English
Weight
887 KB
Volume
28
Category
Article
ISSN
0172-8083

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Cloning and characterization of a chitin
✍ Irene García; José M. Lora; Jesús Cruz; Tahía Benítez; Antonio Llobell; José A. 📂 Article 📅 1994 🏛 Springer-Verlag 🌐 English ⚖ 969 KB

A cDNA of Trichoderma harzianum (chit42), coding for an endochitinase of 42 kDa, has been cloned using synthetic oligonucleotides corresponding to amino-acid sequences of the purified chitinase. The cDNA codes for a protein of 423 amino acids. Analysis of the N-terminal amino-acid sequence of the ch