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Primary culture of microvascular endothelial cells from canine meniscus

✍ Scribed by Dr. Raymond R. Miller; Pamela A. Rydell

Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
488 KB
Volume
11
Category
Article
ISSN
0736-0266

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Microvascular endothelial cells were isolated and cloned from canine knee menisci. Intitially, the endothelial cell colonies in culture exhibited a cobblestone morphology. With passage and time, these cultures adopted a fibroblastic or fusiform morphology. The endothelial nature of the cells was demonstrated by positive immunostaining with antibodies to von Willebrand factor, laminin, and type‐IV collagen and by capillary‐like tube formation on Matrigel. These endothelial cells could be passaged as many as 10 times with retention of the endothelial characteristics. This population of meniscal microvascular endothelial cells will facilitate studies on the role of blood vessels in meniscal repair.

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