Prilocaine–cyclodextrin–liposome: effect of pH variations on the encapsulation and topology of a ternary complex using 1H NMR
✍ Scribed by Luís Fernando Cabeça; Isis Martins Figueiredo; Eneida de Paula; Anita Jocelyne Marsaioli
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 145 KB
- Volume
- 49
- Category
- Article
- ISSN
- 0749-1581
- DOI
- 10.1002/mrc.2740
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✦ Synopsis
Abstract
A better comprehension of the prilocaine (PLC)–β‐cyclodextrin (β‐CD) complex liberation to membranes was provided by studying the architectural supramolecular arrangements of PLC, β‐CD and egg phosphatidylcholine (EPC) liposomes, a membrane model. The topologies and possible interactions of mixtures of PLC, β‐CD and EPC liposomes were investigated by nuclear magnetic resonances combining experimental ^1^H‐NMR (1D ROESY, STD and DOSY) at different pHs. The results indicate that in the mixture PLC/β‐CD/EPC at pH 10 the PLC molecules are almost totally embedded into the liposomes and little interaction was observed between PLC and β‐CD. However, at pH 5.5 not only was PLC imbedded in the EPC bilayer, but PLC was also interacting with β‐CD. These results were rationalized as a spontaneous PLC release from β‐CD to liposomes vesicles, whereas the PLC/EPC complex formation was higher at pH 10 than pH 5.5. Copyright © 2011 John Wiley & Sons, Ltd.