Prevention of tissue calcification on bioprosthetic heart valve by using epoxy compounds: A study of calcification testsin vitro andin vivo
✍ Scribed by Tingfei, Xi ;Jiazhen, Ma ;Wenhua, Tian ;Xuehui, Lei ;Shuhui, Long ;Baoshu, Xi
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 635 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0021-9304
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✦ Synopsis
Calcification is the principal cause of the clinical failures of the bioprosthetic heart valves fabricated from glutaraldehyde pretreated porcine aortic valves or bovine pericardium. In this paper, we compared t h e calcification o n v a r i o u s t y p e s of bovine pericardiums pretreated with two hydrophilic epoxy compounds adding GA p o s t -t r e a t m e n t (EP 1 a n d EP 2), glutaraldehyde (GA)and nontreated pericardium (Fresh), respectively, by in uitro and in uiuo tests. Significant decrease of calcification was found by pretreatment with both epoxy compounds rather than w i t h g l u t a r a l d e h y d e : 0.250 5 0.001 (Fresh), 0.276 t 0.058 (EP l), 0.302 -C 0.071 (EP 2), and 0.478 t 0.172 (GA) /*.g (Ca)/mg (dried tissue), respectively, after 20 days dipping in a simulating serum solution in uitro; 115.13 -C 60.11 (Fresh), 129.84 -C 51.08 (EP 1), 167.39 2 20.81 (EP 2), and 205.19 2 16.86 (GA) pg/mg, respectively, a f t e r 3 months subcutaneous implantation i n rabbits. The in uifro method for evaluating calcification designed by u s gave t h e similar order among four samples with that obtained by in uiuo test. Because the bovine pericardium pretreated with the e p o x y c o m p o u n d s a d d i n g GA p o s ttreatment possesses the greater tenacity t h a n that pretreated only w i t h epoxy compounds or GA, meanwhile the calcification is also significantly decreased with this pretreatment, it may be expected that the bovine pericardium with this pretreatment will have the greater anticalcification and durability in dynamic stress.