Prevention of nuclear fusion and mitosis and inhibition of desoxyribonuclease by d-usnic acid

✍ Scribed by Marshak, Alfred ;Fager, Jean

• Publisher: Wiley (John Wiley & Sons)
• Year: 1950
• Tongue: English
• Weight: 594 KB
• Volume: 35
• Category: Article
• ISSN: 0095-9898
• DOI: 10.1002/jcp.1030350303

✦ Synopsis

I 1 1 a previous report (Yarshak and Harting, 'AS), it was shown that although d-uwic acid has little or no effect on respiration of fertilized eggs of Arbacia, it mill inhibit cell cleavage and uptake of P31 froni sea water. The present paper gives results of further investigation of its mode of action.

I . Yrcuerztion o f mtclecir fzrsiopt followi+zg fwtilkatiovc lisnic acid as a colloidal suspension in sea water and gelatiiie ~v c r c added to Arbacia eggs in sea water to give a final conccntratiori of 10 p g uxiiic acid and 0.1 ing gelatine per milliliter.

Five minutes later sperm was added. To the controls only gelatine and sperm .vei-e adcied. The fertilized eggs were then fixed at, various intervals in 5% citric acid. The timing of the intervals f o r fixation mas dctcimiiied by examiiiation of controls in the living condition.

illethotls f o r fi.mtiotz c m 7 staining

At the appyopriate time, the sea water was siphoned off' leaving only a thin layer covering the eggs. The suspension w a s then transferred to 2 3 or ,500 1111 e1-1eumeyer flasks where, * Division of Tuberculosis, U. 8. Public Health Scrvice.