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Prevention of Hemolysis of Large Volumes of Red Blood Cells Slowly Frozen and Thawed in the Presence of Dimethylsulfoxide

โœ Scribed by Charles E. Huggins


Book ID
114752531
Publisher
Elsevier Science
Year
1963
Tongue
English
Weight
821 KB
Volume
3
Category
Article
ISSN
0372-1248

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Eight-cell mouse embryos were frozen by using glycerol, ethylene glycol, or erythritol as the cryoprotectant. The samples were slowly cooled (0.5"C/min) to temperatures between -10 and -79ยฐC before direct transfer to liquid nitrogen (-196ยฐC) and thawed slowly ( -20"C/min) or rapidly ( -500ยฐC/min). T